Authors
K El-Shazly, A.M Nour, A.R.Abou Akkada
Publication date
1975
Journal
Analyst
Volume
100
Issue
1189
Pages
263-268
Publisher
Royal Society of Chemistry
Description
The work described is an attempt to develop a reliable method for determining AEP when mixed with related substances from micro-organisms in the rumen. Hydrochloric acid hydrolysates of rumen contents, rumen bacteria, rumen ciliate protozoa and clarified rumen contents were applied to a column (10 × 1 cm) of Dowex 50–X8, eluted with 0·6 N hydrochloric acid and 2·4-ml fractions collected. Inorganic phosphorus was separated in column fractions 2–10 and AEP appeared in fractions 11–23.When fractions containing AEP were spotted on to Whatman No. 1 filter-paper strips the developed chromatograms showed six ninhydrin-positive spots in addition to that of AEP, which was the slowest acid to migrate (RF= 0·3). The substance giving spot No. 4, another phosphonic acid (RF= 0·59), was present in ciliate protozoa and ciliate-free fractions of rumen contents, whereas AEP was confined to protozoa. A highly …
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Scholar articles
K El-Shazly, AM Nour, ARA Akkada - Analyst, 1975
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